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Processed Tissue

01. DSAEK//Descemet’s Stripping Automated Endothelial Keratoplasty

Variations of DSAEK

1. DSAEK using a microkeratome that provides a planar cut offering a smoother posterior stromal bed.

2. DSAEK using a Moria™ microkeratome between 100 -150 microns and 9.5-10.5 mm bed size.

02. DMEK//Descemet’s Membrane Endothelial Keratoplasty

Tissue prepared adhering to strict aseptic technique using validated procedures in either a certified ISO 5 clean room or laminar flood hood. There are 4 main methods used when preparing tissue:

Beginning near the trabecular meshwork, approximately 80-90% of Descemet’s membrane and endothelium is peeled toward the periphery leaving a hinge. A sclera notch is made to indicate the location of the hinge. After being peeled, the DM is carefully placed back into its original position and examined using slit lamp and specular microscopes.

Beginning near the trabecular meshwork, the DM is peeled toward the center leaving a central attachment. The tissue from the 8.5 mm central zone to the limbus is cleared away. One benefit is that the tissue is already trephinated for the surgeon.

Peripheral Hinge preparation is used and while the DM is peeled half-way, a trephine is used to create a “stromal plug.” A S-Stamp orientation mark is then made through the stromal plug onto the anterior DM providing the surgeon with an orientation mark allowing correct orientation during implantation.

Handling Recommendations

Peripheral Hinge and S-Stamp
– Carefully remove cornea from chamber, add Trypan Blue to visualize edges.
– Dry edges with surgical spear being careful not to touch endo.
– Trephinate and remove ring of DM outside trephination.
– Hold cornea opposite of notch and carefully lift outer edge, freeing DM.

Central Hinge
– Carefully remove cornea from chamber, add Trypan Blue to visualize edges.
– Carefully grasp edge and lift peel towards opposite side.

If you don’t see your preferred method, please contact us.

Surgeon Advantages

+ Careful and knowledgeable cornea evaluation using slit lamp, specular microscopy and Optical Coherence Tomography (OCT) before and after processing. + Thorough disease testing / screening. + Competent and experienced staff + Safe and reliable distribution delivery system + Rapid procurement and release of tissue that is among the best of US eye banks. + On-line surgery scheduling. + Collaborations with centers of excellence throughout the world. + Multiple variations of preparation methods for DSAEK and DMEK. + Environmentally controlled processing theater rooms.